Assessing lysosomal dysfunction in iPSC derived microglia
PhD Internship - (Neuroscience/Glial biology) - RiSE (9 months)
Who we are
At Roche, 98.000 people across 150 countries are pushing back the frontiers of healthcare. Working together, we`ve become one of the world`s leading research focused healthcare groups. Our success is built on innovation, curiosity and diversity, and on seeing each other`s differences as an advantage. To innovate healthcare, Roche has ambitious plans to keep learning and growing – and is seeking people who have the same goals for themselves.
At Roche, we believe it’s urgent to deliver medical solutions right now – even as we develop innovations for the future. We are passionate about transforming patients’ lives and we are fearless in both decision and action. And we believe that good business means a better world.
That is why we come to work every single day. We commit ourselves to scientific rigour, unassailable ethics, and access to medical innovations for all. We do this today to build a better tomorrow.
The RiSE program (Roche Internships for Scientific Exchange) is a highly competitive student research program at Roche. It offers the most talented postgraduate PhD and medical degree students the opportunity to be fully integrated into our interdisciplinary and international industry R&D environment. As a RiSE student you will enhance your competencies, gain valuable work experience with us, and eventually become part of a world-wide network of RiSE Alumni.
The Neuroscience and Rare Diseases Discovery and Translational Area (NRD DTA) is developing medicines for a range of serious neurological diseases, including multiple sclerosis, Alzheimer’s disease, Parkinson’s disease, autism, spinal muscular atrophy, and Huntington’s disease.
As RiSE student in the NRD DTA you will work collaboratively between the Lysosomal Biology group and Neuroimmunology labs, at the Roche Innovation Center Basel, Switzerland. The project aims to elucidate the mechanisms of lysosomal dysfunction in myeloid cells in the context of neurodegenerative disease. You will be working with human induced pluripotent stem cells (hiPSCs), differentiate them into microglia, and assess the impact of the certain genetic variants on various cellular phenotypes. In addition, state of the art technologies to purify lysosomes will be employed to study lysosomal function with biochemical methods. You will be mentored by a Roche scientist, who will both supervise and collaborate with you on the project, and provide you with the needed work infrastructure and collaborative network across the neuroscience research community within Roche.
During the internship, your tasks will include:
- Culturing, differentiation, and characterization of isogenic iPSC lines with genetic variants into microglia/macrophages
- Performing functional assays to elucidate the impact of genetic variants on lysosomal function, phagocytosis, and lipid processing
- Applying pharmacological or genetic perturbations to probe cellular pathways associated with these phenotypes
- Performing lyso IPs to explore changes in lysosomal enzymes associated with microglial function
- Regularly presenting your findings in lab meetings and department seminars
- Contributing to a manuscript and external presentations that contain the results of this research
Who You Are
You’re someone who wants to influence your own development. You’re looking for a company where you have the opportunity to pursue your interests across functions and geographies. Where a job title is not considered the final definition of who you are, but the starting point.
Moreover you are/have:
- Enrolled in a PhD or medical degree program at a university and are looking to expand your experience with an industry internship
- Proficient in maintaining and treating iPSC/ESC derived culture systems
- Background in cellular neurobiology, neuroimmunology, or other related subject
- Proven track record in cell culture, cellular assays, and biochemistry (dose response treatments, fluorescent or confocal microscopy, IPs / Western blot, etc.)
- Independent, proactive, and willing to work in a highly collaborative and dynamic research environment
Information about application documents and start date:
The preferred start date of the internship is February 2022 or upon availability. Please clearly indicate your preferred starting date of internship on your motivation letter.
Applications need to include a CV and a cover letter, as well as a letter from your academic supervisor supporting your application to the RiSE Program.
Please note that due to regulations non-EU/EFTA citizens have to provide a certificate from the university stating that an industry internship is mandatory as part of the university training.
Do you know what Roche stands for? Roche embraces diversity and equal opportunity in a serious way. We are committed to building a team that represents a variety of backgrounds, perspectives, and skills. The more inclusive we are, the better our work will be.
If you still have questions, then please check our FAQs and videos on careers.roche.ch/faq
Job Level:Entry Level